Polymerase Chain Reaction (PCR) is a molecular biology technique that enables the amplification of specific DNA sequences.
The Pan Bacterial PCR (Polymerase Chain Reaction) test is a powerful molecular diagnostic tool used to identify a wide range of bacterial pathogens in clinical specimens. This test is particularly valuable in situations where traditional culture methods may fail or be too time-consuming. By amplifying and detecting bacterial DNA, the Pan Bacterial PCR test provides rapid and accurate results, facilitating timely diagnosis and treatment of bacterial infections.
1] Sample Collection - The test can be performed on various types of clinical samples, including Blood, Cerebrospinal fluid (CSF), Sputum, Urine, Wound swabs.
2] Laboratory Process -
A] DNA Extraction: The first step involves extracting bacterial DNA from the clinical sample. This process removes contaminants and isolates the nucleic acids necessary for PCR.
B] PCR Amplification: Universal primers that target conserved bacterial genes (such as 16S rRNA) are added to the extracted DNA. The PCR amplification process begins, resulting in the production of millions of copies of the target bacterial DNA.
C] Detection: After amplification, the presence of amplified DNA is detected using techniques such as gel electrophoresis, real-time PCR, or specific probes. This step confirms the presence of bacterial DNA in the sample.
The results are interpreted based on the presence or absence of amplified DNA. Positive results indicate the presence of bacterial pathogens, while negative results suggest that no detectable bacterial DNA was present in the sample.
At Diagnopein, we offer comprehensive, accurate, and timely diagnostic services, including the Pan Bacterial PCR Test. With our NABL-accredited labs, cutting-edge technology, and skilled technicians, you can be assured of reliable results every time. Get your results quickly, especially when time is crucial in detecting severe infections.
Competitive pricing without compromising on quality. Our team of experienced professionals ensures the test is conducted smoothly with minimal discomfort. We provide not just the Pan Bacterial PCR test results but insights into your health condition, helping you and your doctor make informed decisions about treatment. For more information call us at +91 9204 108108.
The test can be performed on various clinical samples, including blood, cerebrospinal fluid (CSF), sputum, urine, and wound swabs. The choice of sample depends on the suspected site of infection.
Yes, limitations include the potential for false positives or negatives, the need for specialized laboratory equipment and trained personnel, and higher costs compared to traditional culture methods.
Yes, the test is safe. The main risk involved is related to blood collection or sample collection procedures, which may cause minor discomfort or bruising.
The test involves extracting bacterial DNA from a clinical sample, amplifying it using PCR with universal primers targeting conserved bacterial genes, and then detecting the amplified DNA. This process allows for the identification of multiple bacterial species simultaneously.
