A corneal button is a disc-shaped section of corneal tissue that is often excised for diagnostic purposes.
This surgical procedure is typically performed in cases where there is a suspected infection, significant corneal damage, or other conditions such as corneal dystrophies. Once removed, the corneal button can be sent for histopathological examination and culture to identify any infectious agents. Culture and sensitivity testing of corneal buttons is a vital diagnostic procedure in ophthalmology.
1] Surgical Removal: The corneal button is surgically excised from the eye, usually under sterile conditions. The procedure is typically performed by an ophthalmologist.
2] Sample Handling: Once removed, the corneal button is placed in a sterile container to prevent contamination. Proper handling is critical to ensure accurate results.
3] Laboratory Culture: The corneal button is sent to a microbiology laboratory, where it is cultured on specific media that support the growth of potential pathogens. This process can take several days, depending on the organism being tested.
4] Sensitivity Testing: Once pathogens are identified, sensitivity testing is performed to determine the effectiveness of various antibiotics or antifungal agents against the isolated organisms. This is typically done using standard methods such as disk diffusion or broth microdilution.
5] Reporting Results: The results of the culture and sensitivity tests pune are compiled into a report that includes the identified organisms and their sensitivities to various antimicrobial agents. This information is crucial for guiding treatment decisions.
Culture and sensitivity testing pune can reveal a variety of infectious agents responsible for corneal infections:
1] Bacteria: The most common bacterial pathogens include:
A] Pseudomonas aeruginosa: Often associated with contact lens wear.
B] Staphylococcus aureus: A common cause of postoperative infections.
C] Streptococcus pneumoniae: Known for causing severe corneal ulcers.
2] Fungi: Fungal infections are less common but can be particularly severe, especially in immunocompromised patients. Common fungal pathogens include:
A] Fusarium species: Frequently associated with corneal ulcers in contact lens wearers.
B] Aspergillus species: Can cause severe corneal infections in certain conditions.
3] Viruses: Viral infections such as herpes simplex virus (HSV) can also affect the cornea, leading to herpetic keratitis. While these infections are typically diagnosed clinically, culture and sensitivity may be used in specific cases.
1] Positive Culture: A positive Culture and Sensitivity (Corneal Button) indicates the presence of infectious organisms. The specific pathogen identified will guide the choice of antibiotic or antifungal therapy.
2] Sensitivity Profile: The sensitivity profile indicates which antibiotics are effective against the identified organism. This information helps ophthalmologists select the most appropriate treatment, minimizing the risk of resistance development.
3] Negative Culture: A negative Culture and Sensitivity (Corneal Button) may indicate that no infectious organism was present, or it could suggest that the pathogen was not detected due to prior antibiotic use or limitations in culture techniques.
At Diagnopein, we offer comprehensive, accurate, and timely diagnostic services, including the Culture and Sensitivity (Corneal Button) test Pune. With our NABL-accredited labs, cutting-edge technology, and skilled technicians, you can be assured of reliable results every time. Get your results quickly, especially when time is crucial in detecting severe infections.
Competitive pricing without compromising on quality. Our team of experienced professionals ensures the test is conducted smoothly with minimal discomfort. We provide not just the Culture and Sensitivity (Corneal Button) test results but insights into your health condition, helping you and your doctor make informed decisions about treatment. For more information call us at +91 9204 108108.
1. Culture Method
2. Sample
3. Colony Count
4. Organism(s) Isolated
5. Culture Report: Culture yields growth of
6. Culture isolated after 7 days :
7. Culture isolated after 14 days:
8. Culture isolated after 21 days:
9. Ampicillin
10. Amikacin
11. Amoxicillin clavulanate
12. cefoperazon+sulbactam
13. Cefuroxime
14. Cefepime
15. Cefotaxime
16. Ciprofloxacin
17. Ertapenem
18. Gentamicin
19. Imipenem
20. Meropenem
21. Norfloxacin
22. Nitrofurantoin
23. Piperacillin-tazobactam
24. Trimethoprim-Sulfamethoxazole (Cotrimoxazole)
A] Diagnose the specific pathogens causing corneal infections. B] Guide the selection of appropriate antimicrobial treatments. C] Assess treatment efficacy and monitor for potential complications.
Once removed, the corneal button is placed in a sterile container and sent to a microbiology laboratory for culture and sensitivity analysis.
The main risks include infection, bleeding, or complications related to the surgical removal of the corneal button. However, these risks are generally low when performed by experienced professionals.
