Cytokeratins are a subset of intermediate filament proteins found in the cytoskeleton of epithelial cells.
The CK cytokeratin test is a laboratory test used to identify and analyze specific cytokeratin proteins in tissue samples. It is commonly performed using immunohistochemical staining techniques, which allow pathologists to visualize the presence and distribution of cytokeratins in biopsy specimens.They are classified into two main categories: type I (acidic) and type II (basic to neutral). There are numerous cytokeratin isoforms, each identified by a unique number (e.g., CK7, CK20). The specific cytokeratins expressed can vary widely depending on the type of epithelial tissue and can be indicative of the tissue's origin.
The primary purpose of the CK cytokeratin test is to aid in the diagnosis and characterization of tumors, particularly:
1] Identifying Tumor Origin: CK expression patterns can help determine the type of epithelial tissue from which a tumor originates, providing clues for its classification.
2] Differentiating Tumor Types: Certain cytokeratin markers are characteristic of specific tumor types, helping to differentiate between benign and malignant lesions.
3] Prognostic Information: The presence or absence of specific cytokeratins can provide information about the aggressiveness of a tumor and its likely clinical behavior.
1] Sectioning: Thin slices of the embedded tissue are cut and placed on microscope slides.
2] Deparaffinization and Rehydration: The slides are treated to remove paraffin and rehydrate the tissue sections.
3] Antibody Staining: Specific antibodies that target the cytokeratin proteins of interest are applied to the tissue sections. These antibodies are often labeled with a dye or enzyme that facilitates visualization.
4] Visualization: After washing away unbound antibodies, the slides are treated with a substrate that reacts with the enzyme or dye, producing a colored product. The slides are then examined under a microscope.
5] Interpretation: A pathologist assesses the staining patterns and intensities, determining which cytokeratins are expressed and their localization within the tissue.
1. Tumor Diagnosis - The CK cytokeratin test is particularly useful in diagnosing various carcinomas. For instance:
A] Lung Carcinomas: CK7 and CK20 can help differentiate between adenocarcinoma and squamous cell carcinoma.
B] Breast Carcinomas: Expression of CKs can assist in identifying breast cancer subtypes, impacting treatment decisions.
C] Gastrointestinal Tract Tumors: CK20 is often expressed in colorectal carcinomas, while CK7 expression can suggest tumors from other sites.
2. Prognostic Assessment - The expression levels of specific cytokeratins may provide prognostic information. For example, high expression of certain CKs in breast cancer may indicate a worse prognosis, while the presence of others may suggest a more favorable outcome.
3. Identifying Tumor Recurrence - The CK cytokeratin test can also be helpful in monitoring for recurrence of tumors. Changes in cytokeratin expression patterns over time can indicate whether a tumor has returned or metastasized.
At Diagnopein, we offer comprehensive, accurate, and timely diagnostic services, including the CK Cytokeratin test Pune. With our NABL-accredited labs, cutting-edge technology, and skilled technicians, you can be assured of reliable results every time. Get your results quickly, especially when time is crucial in detecting severe infections.
Competitive pricing without compromising on quality. Our team of experienced professionals ensures the test is conducted smoothly with minimal discomfort. We provide not just the CK Cytokeratin test results but insights into your health condition, helping you and your doctor make informed decisions about treatment. For more information call us at +91 9204 108108.
Yes, certain patterns of cytokeratin expression can offer insights into the aggressiveness of a tumor and its likely clinical behavior.
The test involves collecting a tissue sample through a biopsy, followed by immunohistochemical staining of the sample to visualize the presence of cytokeratins.
Yes, the test can yield false positives or negatives, and variability in cytokeratin expression may complicate interpretation.
Yes, it is a well-established test in pathology laboratories and is routinely used to aid in the diagnosis of epithelial tumors.
