The TB PCR (Polymerase Chain Reaction) DNA test is a molecular diagnostic method that detects the genetic material (DNA) of the Mycobacterium tuberculosis bacteria.
Unlike traditional TB tests, such as the Mantoux tuberculin skin test or sputum smear microscopy, which may take time and offer limited accuracy, the TB PCR DNA test provides rapid and highly sensitive detection of TB bacteria, even in very small quantities.
A] Sample Collection:
1] Pulmonary TB: Sputum samples are the most common for diagnosing pulmonary TB. The patient provides a sputum sample, which is collected in a sterile container.
2] Extrapulmonary TB: For cases of extrapulmonary TB, other samples may be collected, including urine, cerebrospinal fluid, or tissue biopsies.
B] Sample Preparation:The collected sample is then processed to extract the DNA of the Mycobacterium tuberculosis bacteria. This process involves using chemicals and enzymes that break down the cells and release the genetic material.
C] PCR Amplification:The extracted DNA is subjected to PCR amplification, where the TB bacterial DNA is copied thousands of times. This makes it easier to detect even small quantities of the bacteria.
D] Analysis:Once the PCR amplification is complete, the sample is analyzed using specialized laboratory equipment. The presence of TB DNA sequences confirms an infection.
E] Result Reporting:The results are reported as either positive or negative for TB, and in some cases, the test may provide additional information regarding drug resistance.
1] Positive Result:A positive TB PCR DNA test indicates that Mycobacterium tuberculosis DNA was detected in the sample, confirming an active TB infection. Depending on the strain, further tests may be conducted to determine whether the infection is drug-resistant.
2] Negative Result: A negative result means that no TB DNA was detected in the sample. However, this does not always rule out TB, especially in cases where the bacterial load is very low. Additional tests may be recommended if TB is still strongly suspected.
At Diagnopein, we offer comprehensive, accurate, and timely diagnostic services, including the TB PCR DNA test. With our cutting-edge technology, and skilled technicians, you can be assured of reliable results every time. Get your results quickly, especially when time is crucial in detecting severe infections.
Competitive pricing without compromising on quality. Our team of experienced professionals ensures the test is conducted smoothly with minimal discomfort. We provide not just the TB PCR DNA test results but insights into your health condition, helping you and your doctor make informed decisions about treatment. For more information call us at +91 9204 108108.
A sample (usually sputum, blood, or other bodily fluids) is collected from the patient and tested in a lab using PCR (Polymerase Chain Reaction) technology. This process amplifies TB DNA, allowing for easy detection.
In most cases, no special preparation is needed. However, your doctor may provide specific instructions depending on the type of sample being collected.
The main advantages include faster results, higher sensitivity, and the ability to detect TB in cases where traditional methods might fail, such as in low-bacterial load samples.
Yes, the TB PCR DNA test can detect drug-resistant strains of TB, helping doctors choose the right course of treatment, especially for multi-drug-resistant TB (MDR-TB).
